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CHEF Mapper A2
Overview
Time gradient: linear, with the FIGE function
The pulsed-field gel electrophoresis system achieves the separation of DNA by alternately altering the electric field between DNA molecules, allowing them to migrate through agarose gel pores at different rates. The CHEF Mapper A series is designed for the detection and separation of DNA molecules within the range of 100 bp to 10 Mb, offering high resolution within this spectrum. It is primarily used in fields such as genomic DNA separation analysis, chromosome DNA isolation, construction, identification, and analysis of large-fragment genomic libraries, transgenic research, and synthetic biology. Each CHEF Mapper A system includes a controller, electrophoresis tank, cooling device, circulating pump, and accessories.
★Advanced Technology: Combines CHEF and PACE pulsed-field techniques to achieve superior results, ensuring straight migration paths without deviation.
★Independent Control: Features 24 platinum electrodes (0.5mm diameter) with individually controllable electrode potentials, each electrode being replaceable independently.
★Unique Algorithm: Employs a distinctive pulsed-field control algorithm, offering enhanced separation efficiency, easily distinguishing linear and circular DNA, with superior separation of large circular DNA.
★Automatic Calculation: Incorporates automatic calculation functionality, integrating the determination of 10 key variables to assist users in achieving optimal experimental conditions.
★Program Storage: Capable of storing 15 complex experimental programs, each consisting of no fewer than 8 program modules.
★Multi-State Vector Variation: Each pulse cycle can reach 10 vectors, allowing the definition of each angle, voltage, and duration.
★Transition Slope: Applies linear, concave, or convex hyperbola functions.
★Automation: In the event of a power interruption, the system automatically records and restarts the electrophoresis.
★Configurability: Users can set conditions independently, with the user manual documenting a wide range of electrophoresis conditions for different separation spans.
★Flexibility: The system can select voltage gradients and transition times specific to DNA size spans.
★Large Screen: 7-inch LCD screen, easy to control, with unique software control for simplified operation.
★Temperature Detection: Dual temperature probes directly detect buffer temperature, with an error margin of <±0.5℃.
★Circulation System: Equipped with a buffer circulation system, precisely controlling and monitoring buffer temperature to ensure constant temperature and ionic balance during electrophoresis.
★High Safety: Polycarbonate transparent safety cover, automatically cutting off power when the cover is lifted to prevent contact.
★Adjustable Level: Electrophoresis tank and rubber mold designed with adjustable support angles for leveling. Mold: The electrophoresis tank features an integrated mold structure, requiring no bonding; the electrode holder is equipped with φ0.5mm platinum electrodes, resistant to deformation and long-lasting, ensuring stable experimental results.
★Pause and Resume: Equipped with pause/resume operation functionality, supporting power outage time recording and intelligent recovery.
★Polymorphism: Possesses multi-state vector variable functionality, allowing up to 10 different pulse angles and duration combinations within a single pulse cycle.
★Pulse Angle: Pulse angles can be freely selected between 0-360°, enabling effective separation of everything from large to small, even plasmid DNA, on the same system.
★Pulse Time Gradient: Features linear and nonlinear (convex and concave) pulse time gradients, with nonlinear gradients providing a broader separation dynamic range, facilitating the determination of fragment sizes.
★Real-Time Monitoring: Displays set parameters and working status simultaneously, compatible with real-time monitoring software for upper-level computers.
★Secondary Pulse Function: Secondary pulses accelerate DNA release from agarose gel, aiding the separation of ultra-large DNA fragments and enhancing resolution.
★PulseNet China Network Compatibility: Connects with the national pathogen surveillance network and PulseNet China monitoring network, distinguishing fragments with similar molecular weights.
◇Voltage Gradient: 0.5V/cm to 9.6V/cm, with increments of 0.1V/cm;
◇Maximum Current: 0.5A;
◇Maximum Voltage: 350V;
◇Pulse Angle: 0° to 360°, with increments of 0.1°;
◇Time Gradient: Linear and Non-linear;
◇Transition Time: 50 milliseconds to 18 hours;
◇Maximum Operating Time: 999 hours;
◇Number of Electrodes: 24, independently controlled;
◇Polymorphic Functionality;
◇Temperature Range: 0℃ to 50℃, with a detection error of less than ±0.5℃.
◇Capable of accommodating up to 2.4 liters of buffer solution.
