CHEF Mapper A4

Pulsed Field Electrophoresis System is the separation of DNA by alternately changing the electric field between pairs of pairs of DNA molecules and passing through the agarose gel pore at different migration rates.

The CHEF Mapper A series is suitable for the detection and isolation of DNA molecules of 100bp-10 Mb, with a high resolution in the range, mainly used for genomic DNA isolation analysis, chromosomal DNA isolation, large fragment genomic library construction, identification and analysis, transgenic research, synthetic biology and other fields.

Each CHEF Mapper A system contains the controller, electrophoresis tank, cooling device, circulating pump and accessories.

● Advanced technology: combined with CHEF, PACE pulse field technology, to achieve good effect, straight lane, not bending.

● Independent control: the electrode potential composed of 24 platinum electrodes (0.5mm diameter) can be controlled independently, and each electrode can be replaced separately.

● Unique algorithm: the use of unique pulse control algorithm, the separation effect is better, can easily distinguish linear and circular DNA, the large ring DNA separation effect is better;

● Unique automatic calculation function, combined with the determination of 10 main variables, to help users to obtain the most ideal experimental conditions;

● Program memory: store 15 complex experimental programs, each program consisting of no less than 8 program modules;

● Multi-state vector variation: up to 10 vectors / pulse period, which can define each angle, voltage and duration;

● Conversion slope: linear, concave or convex applied hyperbolic function;

● Automation: If the system is interrupted due to a power failure, it can automatically record and restart the electrophoresis.

● Settable: the user can set the conditions by itself, and the user manual records a large number of electrophoresis conditions under different separation spans.

● Flexible: the system can select the voltage gradient and conversion time of a specific DNA size span.

● Large screen: 7-inch LCD screen, easy to control, unique software control, easy to operate.

● Temperature detection: double temperature probe, direct detection of buffer temperature, error <± 0.5℃.

● Circulation device: equipped with a buffer circulation system, it can accurately control and monitor the temperature of buffer solution and ensure constant temperature and ion balance during electrophoresis.

● High safety: polycarbonate transparent glass safety cover, when the cover is lifted, automatically cut off the power supply, to prevent contact;

● Adjuzeable level: electrophoresis tank and rubber maker design has adjustable support angle, adjustable level.

● Mold: the electrophoresis tank adopts integrated integral mold structure, no bonding; electrode frame with φ 0.5mm platinum electrode, not easy to deform and long life, ensuring stable experimental effect.

● Pause recovery: with pause / restore operation function, with power off time record and intelligent recovery function.

● Polymorphism: with the vector variable function of multiple states, a single pulse period can run up to 10 different pulse angles and running time combinations;

● Pulse Angle: Pulse Angle can be selected freely between 0-360°, so that users can achieve effective separation of large to small to plasmid DNA on the same system.

● There are linear and nonlinear (convex and concave) pulse time gradients, the nonlinear gradient can provide a wider separation dynamic range, allowing the user to determine the size of the separated fragments.

● Real-time monitoring: display the setting parameters and working conditions at the same time, can be matched with real-time monitoring upper computer software.

● Secondary pulse function: Secondary pulse can accelerate the release of DNA from the agarose gel, thus facilitating the separation of very large DNA fragments and improving the resolution.

● Compatible PulseNet China network: it can be connected with the national pathogenic bacteria monitoring network and PulseNet China monitoring network, and the fragments with similar molecular weight can be distinguished.

● Voltage gradient: 0.5V / cm-9.6V / cm, increment of 0.1V / cm;

● Maximum current: 0.5A;

● Maximum voltage: 350V;

● Pulse angle: 0-360°, 0.1° increments;

● Time gradient: linear and nonlinear;

● Conversion time: 50 ms to 18h;

● Maximum running time: 999h;

● Number of electrodes: 2 4, independent control;

● Polymorphic function;

● Temperature range: 0℃ ~ 50℃, detection error <± 0. 5℃.

● Up to 2.4L of the buffer solution